CD34 Antikörper (FITC)

Produktnummer ABIN120286

Dieser Maus Monoklonal Antikörper erkennt spezifisch CD34 in FACS. Er zeigt eine Reaktivität gegenüber Hund.

Kurzübersicht für CD34 Antikörper (FITC) (ABIN120286)

Target: CD34

Reaktivität: Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 1H6

Produktdetails anti-CD34 Antikörper (FITC)

Spezifität: SM2257F is specific for Canine CD34, a cell surface antigen of approximately 110 kDa expressed by endothelial cells and haematopoietic stem cells. Clone 1H6 is reported for use in CD34+ enrichment studies (4,5).

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Canine CD34 fusion protein. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (neat - 1/10): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gen-ID: 415130

NCBI Accession: NP_001003341

UniProt: Q28270